This kit is based on real-time PCR fluorescence technology to
detect the COVID-19 nucleic acid
of patients with Flu-like symptoms, patients with severe pneumonia
or patients with asymptomatic infection.
The operators should have professional training of gene
amplification or molecular biologicalmethod detection,
have the relevant experimental operation qualification, and the
laboratory shallhave reasonable biological safety
prevention facilities and protection procedures.
Applicable instruments
A real-time PCR instrument containing at least four colors
fluorescence channels, such as SLAN-
96P, ABI 7500, Roche Light Cycle 480.
Sample requirement
1. Applicable sample type: Throat swab (Oropharyngeal swab/Nasal
swab)
2. Sample collection
2.1 Sample time: The reagent is not affected by the specific
clinical symptoms of patients, and
can be sampled at each stage of the course of the disease.
2.2 The test performance of the reagent is not affected when using
the drugs described in the
analysis performance evaluation, and the sample can be taken
normally. It is better to take
samples before using other unverified drugs
2.3 Throat swab requirement: It’s better to use long handle swab
with polypropylene fiber head
or wood handle swab, please select the preservation solution
suitable for cells and viruses.
It is better to directly select the commercial throat swab sampling
tube.
3. Sampling method:
3.1 Oropharyngeal swab: Wipe both tonsils and the posterior
pharyngeal wall with a swab with
moderate force to avoid touching the tongue.
3.2 Nasal swab: Use the swab to penetrate thenasalcavityfor2-3 cm,
and rotateitfor2-3times.
Preservation and delivery of samples
The samples for detection should be stored at under the following
conditions: less than 24 hours
at 2-8℃, less than 1 month at -20℃, or a long time at -70℃. And
repeated freezing and thawing
shall be avoided (freezing and thawing shall not exceed 3 times).
An ice transfer box was used for specimen transportation.
